US 12,128,399 B2
High-throughput crystallographic screening device and method for crystalizing membrane proteins using a sub physiological resting membrane potential across a lipid matrix of variable composition
Jose Lasalde-Dominicci, San Juan, PR (US); Orestes Quesada-Gonzalez, Canovanas, PR (US); Josue Rodriguez-Cordero, Carolina, PR (US); Carlos Baez-Pagan, Carolina, PR (US); and Martin Montoya-Zavala, San Juan, PR (US)
Assigned to UNIVERSITY OF PUERTO RICO, San Juan, PR (US)
Filed by UNIVERSITY OF PUERTO RICO, San Juan, PR (US)
Filed on Jul. 25, 2023, as Appl. No. 18/225,981.
Application 18/225,981 is a continuation of application No. 17/741,595, filed on May 11, 2022, granted, now 11,717,819.
Application 17/741,595 is a continuation of application No. 15/996,946, filed on Jun. 4, 2018, granted, now 11,440,001, issued on Sep. 13, 2022.
Application 15/996,946 is a continuation of application No. 15/638,276, filed on Jun. 29, 2017, abandoned.
Claims priority of provisional application 62/516,860, filed on Jun. 8, 2017.
Claims priority of provisional application 62/356,266, filed on Jun. 29, 2016.
Prior Publication US 2023/0364601 A1, Nov. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C30B 7/12 (2006.01); B01J 19/00 (2006.01); B01J 19/18 (2006.01); B01L 3/00 (2006.01); B01L 3/06 (2006.01); C07K 1/30 (2006.01); C30B 29/58 (2006.01); C40B 40/10 (2006.01)
CPC B01L 3/06 (2013.01) [B01J 19/0046 (2013.01); B01J 19/1893 (2013.01); B01L 3/5085 (2013.01); C07K 1/306 (2013.01); C30B 7/12 (2013.01); C30B 29/58 (2013.01); C40B 40/10 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00522 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00639 (2013.01); B01J 2219/00725 (2013.01); B01J 2219/00745 (2013.01); B01J 2219/00756 (2013.01); B01L 2300/069 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of crystallizing one or more membrane proteins, said method comprising the step of incubating a membrane protein sample in a system, wherein the system comprises:
a sample unit comprising:
a sample holding layer having a plurality of holding wells, wherein each holding well comprises a pair of electrodes, and
a lid layer provided over said plurality of holding wells to seal the contents of said plurality of holding wells from each other; and
a variable direct current potential source electrically coupled to the pair of electrodes of each holding well, wherein the incubation of the membrane protein sample provides crystallization of the one or more membrane proteins.