US 12,128,257 B2
Extracts of Isochrysis sp
Martina Herrmann, Hameln (DE); Holger Joppe, Dassel (DE); Paolo Pertile, San Pietro Viminario (IT); and Lorenzo Zanella, Venezia-Mestre (IT)
Assigned to Symrise AG, Holzminden (DE)
Filed by SYMRISE AG, Holzminden (DE)
Filed on Sep. 16, 2019, as Appl. No. 16/571,882.
Application 16/571,882 is a division of application No. 12/568,475, filed on Sep. 28, 2009, granted, now 10,463,891.
Claims priority of provisional application 61/101,228, filed on Sep. 30, 2008.
Prior Publication US 2020/0222730 A1, Jul. 16, 2020
Int. Cl. A61Q 19/04 (2006.01); A61K 8/9706 (2017.01); A61K 8/9711 (2017.01); A61K 8/9789 (2017.01); A61Q 7/00 (2006.01); A61Q 7/02 (2006.01); A61Q 19/02 (2006.01)
CPC A61Q 19/04 (2013.01) [A61K 8/9706 (2017.08); A61K 8/9711 (2017.08); A61K 8/9789 (2017.08); A61Q 7/00 (2013.01); A61Q 7/02 (2013.01); A61Q 19/02 (2013.01)] 10 Claims
 
1. A method for increasing growth of human hair in a human in need thereof, consisting essentially of:
(i) cultivating Tahitian Isochrysis sp. cells;
(ii) harvesting the cells to obtain completely or substantially intact cell material;
(iii) extracting the completely or substantially intact cell material with a liquid extractant selected from the group consisting of hexane, ethyl acetate, ethanol, water, methanol, isopropanol, and mixtures thereof for up to 24 h at a temperature of not more than 50° C. to provide an extracted cell material;
(iv) separating the extract from the extracted cell material;
(v) applying topically to the hair of the human in need thereof, a therapeutically effective amount of the extract of Tahitian Isochrysis sp. to increase said growth of the human hair, wherein the extract of Tahitian Isochrysis is obtained by the method consisting essentially of the above steps; and
(vi) increasing said growth of human hair in said human in need thereof.