US 12,128,084 B2
Use of PAYCS in regulation of intestinal flora, metabolites, and brain neurotransmitters
Tiantian Zhao, Guangzhou (CN); Yehui Zhang, Guangzhou (CN); Yousheng Zhang, Guangzhou (CN); Wenjuan Jiao, Guangzhou (CN); Weifeng Liu, Guangzhou (CN); and Shuai Chen, Guangzhou (CN)
Assigned to Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou (CN)
Filed by Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou (CN)
Filed on Feb. 16, 2023, as Appl. No. 18/170,163.
Claims priority of application No. 202210147703.1 (CN), filed on Feb. 17, 2022.
Prior Publication US 2023/0330177 A1, Oct. 19, 2023
Int. Cl. A61K 38/08 (2019.01); A61P 1/14 (2006.01); A61P 25/28 (2006.01); A61P 29/00 (2006.01); C12P 21/06 (2006.01)
CPC A61K 38/08 (2013.01) [A61P 1/14 (2018.01); A61P 25/28 (2018.01); A61P 29/00 (2018.01); C12P 21/06 (2013.01)] 6 Claims
 
1. A method for regulating intestinal flora, metabolites, and brain neurotransmitters, comprising a step of administering a peptide to a subject to promote targeted oxidation, improve inflammatory stress, and regulate intestinal microorganism-metabolite-brain neurotransmitter axis; wherein the peptide is PAYCS (SEQ ID NO: 1) and is prepared by using a process including the following conditions for enzymatic hydrolysis by protease:material-liquid ratio 1:3, enzyme dosage 1.3% by weight of the material, pH 7.2, reaction temperature 60° C., and reaction time 50 min; and the conditions for enzymatic hydrolysis using an alkaline protease includes an enzyme dosage of 0.32%, a pH value of 7.3, a reaction temperature of 63° C., and a reaction time of 77 min.