US 12,128,082 B2
Method for extracting flavone aglycones in chrysanthemum morifolium, extract obtained thereby and anti-inflammatory pharmaceutical composition
Hsin Jan Yao, Taixi Township (TW); Yu-Wen Chen, Zhubei (TW); Chu-Hsun Lu, Hsinchu (TW); I-Hong Pan, Zhubei (TW); Wen-Yin Chen, Tainan (TW); Tsung-Lin Yang, Tainan (TW); and Angela Goh, Hsinchu (TW)
Assigned to INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE, Hsinchu (TW)
Filed by INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE, Hsinchu (TW)
Filed on Dec. 23, 2021, as Appl. No. 17/560,487.
Prior Publication US 2023/0201291 A1, Jun. 29, 2023
Int. Cl. A61K 36/287 (2006.01); A61P 29/00 (2006.01)
CPC A61K 36/287 (2013.01) [A61P 29/00 (2018.01); A61K 2236/31 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/53 (2013.01); A61K 2236/55 (2013.01)] 18 Claims
 
1. A method for extracting flavone aglycones in Chrysanthemum morifolium, comprising:
(a) immersing a Chrysanthemum morifolium raw material in water or an aqueous solution to perform an immersion procedure for 3.5 hours or more to obtain an immersion sample,
wherein
the Chrysanthemum morifolium raw material comprises at least one of the following parts of Chrysanthemum morifolium:
a whole plant, a root, a stem, a leaf, and a flower,
a weight ratio of the Chrysanthemum morifolium raw material to the water or aqueous solution is 1:10-35,
the immersion procedure is performed at 20 to 70° C.,
the water or aqueous solution has a pH value of 3.0 to 9.5, and
the immersion sample comprises an immersed Chrysanthemum morifolium raw material and an immersion solution; and
(b) adding an extraction solvent to the immersion sample to perform an extraction procedure for 5-60 minutes to obtain an extract solution,
wherein
a weight ratio of the Chrysanthemum morifolium raw material to the extraction solvent is 1:10-35,
the extraction solvent comprises methanol, ethanol or ethyl acetate,
the extraction procedure is performed at 15 to 50° C., and
the extract solution contains flavone aglycones.