US 12,128,081 B2
Process of obtaining enriched fractions of assets from the artemisia genus, enriched fractions and bioactive composition
Maria Angela De Almeida Meireles Petenate, Campinas (BR); Renata Vardanega, Campinas (BR); and Gislaine Chrystina Nogueira De Faria, Campinas (BR)
Assigned to UNIVERSIDADE ESTADUAL DE CAMPINAS, Campinas (BR); and S DISTRIBUIDORA DE COSMETICOS, Campinas (BR)
Appl. No. 17/418,640
Filed by UNIVERSIDADE ESTADUAL DE CAMPINAS, Campinas (BR); and S DISTRIBUIDORA DE COSMETICOS, Campinas (BR)
PCT Filed Dec. 22, 2019, PCT No. PCT/BR2019/050559
§ 371(c)(1), (2) Date Jun. 25, 2021,
PCT Pub. No. WO2020/132730, PCT Pub. Date Jul. 2, 2020.
Claims priority of application No. 10 2018 077171-0 (BR), filed on Dec. 26, 2018.
Prior Publication US 2022/0080012 A1, Mar. 17, 2022
Int. Cl. A61K 36/00 (2006.01); A61K 8/9789 (2017.01); A61K 36/282 (2006.01); A61Q 19/00 (2006.01)
CPC A61K 36/282 (2013.01) [A61K 8/9789 (2017.08); A61Q 19/00 (2013.01); A61K 2236/37 (2013.01); A61K 2236/39 (2013.01); A61K 2800/10 (2013.01)] 7 Claims
 
1. A process of obtaining a fraction enriched from the Genus Artemisia comprising sequential extractions comprising the stages of:
a) conducting a CO2 extraction at a temperature of 57° C. to 65° C. and a pressure of 50 to 150 bar to obtain a first enriched fraction;
b) conducting sequential extraction of a residue of the first enriched fraction obtained from step a) with CO2 at a temperature of 47° C. to 55° C. and a pressure of 250 to 350 bar to obtain a second enriched fraction; and
c) conducting sequential extraction of a residue obtained from step b) with CO2 at a temperature of 47° C. to 55° C. and a pressure of 250 to 350 bar to obtain a residual fraction
wherein a S/F (solvent to feed) ratio of step c) is 5 to 7 times greater than the S/F ratio of step b).