	US 12,127,553 B2
	Cell cryopreservation composition and cryopreservation method
Asuka Nogami, Kashiwara (JP); Motoki Tatsumi, Kashiwara (JP); Nanase Ishii, Kashiwara (JP); Mizuyuki Ryu, Kashiwara (JP); Yoshihiko Hirata, Kashiwara (JP); Yoshiki Sawa, Suita (JP); Shigeru Miyagawa, Suita (JP); Atsuhiro Saito, Suita (JP); and Hirotatsu Ohkawara, Suita (JP)
Assigned to SARAYA CO., LTD., Osaka (JP); and OSAKA UNIVERSITY, Osaka (JP)
Filed by Saraya Co., Ltd., Osaka (JP); and Osaka University, Suita (JP)
Filed on May 19, 2022, as Appl. No. 17/664,194.
Application 17/664,194 is a division of application No. 16/482,236, abandoned, previously published as PCT/JP2018/002879, filed on Jan. 30, 2018.
Claims priority of application No. 2017-016266 (JP), filed on Jan. 31, 2017.
Prior Publication US 2022/0272963 A1, Sep. 1, 2022
Int. Cl. C12N 1/04 (2006.01); A01N 1/02 (2006.01); C12N 5/0775 (2010.01)

CPC A01N 1/0221 (2013.01) [C12N 1/04 (2013.01); C12N 5/0663 (2013.01); C12N 5/0665 (2013.01)]

8 Claims

1. A cell cryopreservation method comprising:

adding a 1 volume % of a composition comprising 0.01 wt % to 20 wt % of a sophorose lipid to cells in a cell culture medium just before or up to 6 hours before cryopreserving the cells; and

cryopreserving the cell culture medium, wherein the composition improves cell viability after cryopreservation compared to cells that are cryopreserved with a similar composition that does not contain the sophorose lipid.