US 12,127,521 B2
Male sterility maintainer line plant and use thereof
Jinsheng Lai, Beijing (CN); Haiming Zhao, Beijing (CN); and Weibin Song, Beijing (CN)
Assigned to CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
Appl. No. 17/618,027
Filed by CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
PCT Filed Jun. 9, 2020, PCT No. PCT/CN2020/095082
§ 371(c)(1), (2) Date Dec. 10, 2021,
PCT Pub. No. WO2020/248969, PCT Pub. Date Dec. 17, 2020.
Claims priority of application No. 201910496386.2 (CN), filed on Jun. 10, 2019.
Prior Publication US 2022/0256793 A1, Aug. 18, 2022
Int. Cl. C12N 15/82 (2006.01); A01H 1/02 (2006.01); A01H 6/46 (2018.01)
CPC A01H 1/023 (2021.01) [A01H 6/4684 (2018.05); C12N 15/8289 (2013.01)] 15 Claims
 
1. An isolated nucleic acid molecule, which comprises a first polynucleotide, a second polynucleotide and a third polynucleotide; wherein the first polynucleotide comprises a nucleotide sequence of a male fertility restoring gene of a plant that has male sterility caused by a male sterility gene; wherein the male sterility gene is a recessive male sterility gene, which causes plant male sterility in a homozygous state; wherein the restoring gene is ms45 and has the nucleotide sequence of SEQ ID NO: 1;
wherein the second polynucleotide comprises: (a) a first screening gene that regulates a seed external trait, and (b) a second screening gene that regulates a plant external trait;
wherein,
(i) the first screening gene is Lc gene, and the second screening gene is Wi2 gene; or
(ii) the first screening gene is interfering RNA of CWI-2 gene, and the second screening gene is Le gene; or
(iii) the first screening gene is interfering RNA of CWI-2 gene, and the second screening gene is Oy1 gene;
wherein the Lc gene has the nucleotide sequence of SEQ ID NO: 4, the Oy1 gene has the nucleotide sequence of SEQ ID NO: 6, the Wi2 gene has the nucleotide sequence of SEQ ID NO: 8, and the nucleotides encoding the interfering RNA of CWI-2 gene has the nucleotide sequence of SEQ ID NO: 18; and
wherein the third polynucleotide comprises a nucleotide sequence of a heterologous selective marker gene.