US 12,456,540 B2
Systems and methods for identifying and expressing gene clusters
Brian Thomas Naughton, Mountain View, CA (US); Colin Harvey, Mountain View, CA (US); Ulrich Schlecht, Falkensee (DE); Maureen Elizabeth Hillenmeyer, Palo Alto, CA (US); and Joe Horecka, Fremont, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Jan. 26, 2021, as Appl. No. 17/158,942.
Application 17/158,942 is a continuation of application No. 16/461,750, abandoned, previously published as PCT/US2017/062100, filed on Nov. 16, 2017.
Application 16/461,750 is a continuation in part of application No. 15/469,452, filed on Mar. 24, 2017, granted, now 10,612,032, issued on Apr. 7, 2020.
Claims priority of provisional application 62/481,601, filed on Apr. 4, 2017.
Claims priority of provisional application 62/423,196, filed on Nov. 16, 2016.
Prior Publication US 2021/0230611 A1, Jul. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G16B 20/00 (2019.01); C12N 15/79 (2006.01); G16B 5/00 (2019.01); G16B 10/00 (2019.01); G16B 25/00 (2019.01); G16B 30/00 (2019.01); G16B 30/10 (2019.01)
CPC G16B 20/00 (2019.02) [C12N 15/79 (2013.01); G16B 5/00 (2019.02); G16B 10/00 (2019.02); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02)] 9 Claims
 
1. A method for producing a small molecule to screen for inhibition of a first target protein, the method comprising:
identifying a fungal protein based on its similarity to a first target protein, wherein the fungal protein has at least 60% sequence identity to the first target protein;
identifying a fungal gene cluster that is within 20,000 bases of a genomic region that encodes the fungal protein;
expressing the fungal gene cluster or a plurality of genes from the fungal gene cluster in a host cell to produce a compound;
isolating the compound produced by the fungal gene cluster or the plurality of genes from the fungal gene cluster; and
screening the isolated compound for inhibition of an activity of the first target protein;
wherein the first target protein is a mammalian protein.