US 12,456,315 B2
Methods for distinguishing particles in a fluid sample
Bernardo Cordovez, San Franciso, CA (US); Paul Dyer, Burlingame, CA (US); Spencer Borg, Burlingame, CA (US); and Adam Ross, Burlingame, CA (US)
Assigned to OPTOFLUIDICS, INC., Philadelphia, PA (US)
Filed by Optofluidics Inc., Philadelphia, PA (US)
Filed on Aug. 16, 2022, as Appl. No. 17/888,936.
Claims priority of provisional application 63/233,643, filed on Aug. 16, 2021.
Prior Publication US 2023/0360413 A1, Nov. 9, 2023
Int. Cl. G06V 20/69 (2022.01); G01N 1/30 (2006.01); G01N 15/01 (2024.01); G01N 15/06 (2024.01); G06V 10/56 (2022.01)
CPC G06V 20/693 (2022.01) [G01N 1/30 (2013.01); G01N 15/0625 (2013.01); G06V 10/56 (2022.01); G06V 20/698 (2022.01); G01N 15/01 (2024.01)] 11 Claims
OG exemplary drawing
 
1. A method for identifying beads in a cell therapy sample comprises:
acquiring a background SIMI image and a background brightfield image of a membrane filter while the membrane filter is free of a fluid sample;
introducing a fluid sample onto the membrane filter;
acquiring a SIMI image and a brightfield image of filtered particles resting on the membrane filter;
distinguishing between the filtered particulates and the membrane filter based on the background SIMI image;
generating a particle mask based on the SIMI image; and
detecting beads via the particle mask.