	US 12,455,290 B2
	Methods for detecting meteorin-β activity
Gerardo Arrevillaga Boni, San Diego, CA (US); Marcela Hernandez Ruiz, San Diego, CA (US); and David Michael Soper, San Diego, CA (US)
Assigned to Biolegend, Inc., San Diego, CA (US)
Filed by BIOLEGEND, INC., San Diego, CA (US)
Filed on Aug. 4, 2021, as Appl. No. 17/394,238.
Application 17/394,238 is a continuation of application No. PCT/US2020/016780, filed on Feb. 5, 2020.
Claims priority of provisional application 62/803,047, filed on Feb. 8, 2019.
Prior Publication US 2022/0034906 A1, Feb. 3, 2022
Int. Cl. G01N 33/68 (2006.01)

CPC G01N 33/6869 (2013.01) [G01N 2333/54 (2013.01)]

15 Claims

1. A method for assessing activity of a meteorin-β polypeptide comprising:

a) contacting a meteorin-β receptor expressing cell with a first composition comprising a co-stimulant, and a second composition comprising a meteorin-β polypeptide;

b) measuring production by the meteorin-β receptor expressing cell of one or more cytokines chosen from chemokine (C-X-C motif) ligand 8 (CXCL8) and interleukin-1 receptor antagonist (IL-1RA), thereby measuring cytokine production; and

c) detecting the activity of the meteorin-β polypeptide in the second composition according to the cytokine production measured in (b).