US 12,455,288 B2
Method of isolating and detecting cancer stem cells
Vincent Carre, Gueret (FR); and Aurélie Lacroix, Panazol (FR)
Assigned to Sylvain Gnaho, Bonneval (FR)
Appl. No. 16/620,235
Filed by CARCIDIAG BIOTECHNOLOGIES, Gueret (FR)
PCT Filed Jun. 1, 2018, PCT No. PCT/FR2018/051280
§ 371(c)(1), (2) Date Dec. 6, 2019,
PCT Pub. No. WO2018/224761, PCT Pub. Date Dec. 13, 2018.
Claims priority of application No. 17/55137 (FR), filed on Jun. 8, 2017; and application No. 17/55139 (FR), filed on Jun. 8, 2017.
Prior Publication US 2021/0080463 A1, Mar. 18, 2021
Int. Cl. G01N 33/574 (2006.01)
CPC G01N 33/57423 (2013.01) [G01N 2333/42 (2013.01)] 14 Claims
 
1. An in vitro method for isolating cancer stem cells of organs involved in respiration in a biological sample comprising:
(a) a step of labelling the cancer stem cells of organs involved in respiration with at least one lectin recognizing the fucose α 1-2 galactose group, said lectin being conjugated to biotin or a fluorophore, to obtain a biological sample in which the cancer stem cells of organs involved in respiration are labelled with at least one lectin,
followed by
(b) a step of isolating said cancer stem cells of organs involved in respiration labelled with at least one lectin,
said isolation step being carried out via a support functionalized with streptavidin or avidin constituted by magnetic beads and in the presence of a magnet, when said lectin is conjugated to biotin,
and said isolation step being carried out by cell sorting in flow cytometry, when said lectin is conjugated to a fluorophore,
wherein said at least one lectin is chosen from the lectins UEA-1 or its homologue TJA-II, ABA, ACA, jacalin, GSL-I and GSL-II.