US 12,455,283 B2
Methods for screening polypeptides capable of binding specific target molecules and tools related thereto
Antti Tullila, Vtt (FI); Tarja Nevanen, Vtt (FI); Henri Arola, Vtt (FI); and Heli Nygren, Vtt (FI)
Assigned to Teknologian tutkimuskeskus VTT Oy, Espoo (FI)
Appl. No. 17/425,534
Filed by Teknologian tutkimuskeskus VTT Oy, Espoo (FI)
PCT Filed Jan. 28, 2020, PCT No. PCT/FI2020/050042
§ 371(c)(1), (2) Date Jul. 23, 2021,
PCT Pub. No. WO2020/157381, PCT Pub. Date Aug. 6, 2020.
Claims priority of application No. 20195053 (FI), filed on Jan. 29, 2019.
Prior Publication US 2022/0214336 A1, Jul. 7, 2022
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54313 (2013.01) 23 Claims
 
1. A method of screening a polypeptide capable of binding a target molecule, wherein the method comprises
i) allowing a capture complex to contact with the polypeptide thereby forming a first complex comprising the capture complex and the polypeptide,
ii) allowing the first complex to contact with a sample comprising target molecules thereby forming a second complex comprising the capture complex, the polypeptide, and a target molecule, and
iii) separating the target molecule of the second complex to a solution, or
i) allowing a polypeptide to contact with a target molecule thereby forming a first complex comprising the polypeptide and the target molecule,
ii) allowing the first complex to contact with a capture complex thereby forming a second complex comprising the capture complex, the polypeptide, and the target molecule, and
iii) separating the target molecule of the second complex to a solution, wherein the polypeptide is an antibody or an antigen binding fragment thereof and the target molecule is a free and intact organic small molecule, wherein the target molecule is not modified with a linker.