	US 12,455,282 B2
	Method and system for measuring analyte concentration in a sample
Kris Covens, Leuven (BE); Sarp Kerman, Leuven (BE); and Willem Van Roy, Leuven (BE)
Assigned to miDiagnostics NV, Heverlee (BE)
Appl. No. 17/636,842
Filed by miDiagnostics NV, Heverlee (BE)
PCT Filed Aug. 21, 2020, PCT No. PCT/EP2020/073504 § 371(c)(1), (2) Date Feb. 18, 2022, PCT Pub. No. WO2021/037725, PCT Pub. Date Mar. 4, 2021.
Claims priority of application No. 19193422 (EP), filed on Aug. 23, 2019.
Prior Publication US 2022/0299503 A1, Sep. 22, 2022
Int. Cl. G01N 33/543 (2006.01); G01N 33/58 (2006.01)

CPC G01N 33/543 (2013.01) [G01N 33/58 (2013.01)]

21 Claims

1. A method of measuring analyte concentration in a sample, comprising:

providing the sample to a microfluidic channel, wherein a surface of the microfluidic channel is provided with affinity probes for binding the analyte to the surface,

temporally measuring a signal which is indicative of the binding of the analyte to the affinity probes,

analyzing the temporally measured signal to determine a pseudo-rate constant which is indicative of how fast the temporally measured signal changes with time, and

determining the analyte concentration in the sample by comparing the determined pseudo-rate constant to a predetermined relationship which relates pseudo-rate constants to analyte concentrations.