US 12,455,276 B2
Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
Amit Meller, Brookline, MA (US); and Meni Wanunu, West Roxbury, MA (US)
Assigned to Trustees of Boston University, Boston, MA (US)
Filed by Trustees of Boston University, Boston, MA (US)
Filed on Apr. 27, 2021, as Appl. No. 17/241,929.
Application 14/815,727 is a division of application No. 12/599,440, granted, now 9,121,843, issued on Sep. 1, 2015, previously published as PCT/US2008/063066, filed on May 8, 2008.
Application 17/241,929 is a continuation of application No. 16/138,658, filed on Sep. 21, 2018, granted, now 11,002,724.
Application 16/138,658 is a continuation of application No. 15/411,782, filed on Jan. 20, 2017, granted, now 10,101,315, issued on Oct. 16, 2018.
Application 15/411,782 is a continuation of application No. 14/815,727, filed on Jul. 31, 2015, granted, now 9,903,820, issued on Feb. 27, 2018.
Claims priority of provisional application 60/928,160, filed on May 8, 2007.
Claims priority of provisional application 60/928,260, filed on May 8, 2007.
Claims priority of provisional application 60/928,158, filed on May 8, 2007.
Prior Publication US 2021/0263011 A1, Aug. 26, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/487 (2006.01); B82Y 15/00 (2011.01); C12Q 1/6869 (2018.01); G01N 21/64 (2006.01)
CPC G01N 33/48721 (2013.01) [B82Y 15/00 (2013.01); C12Q 1/6869 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01); Y10T 428/2982 (2015.01); Y10T 436/143333 (2015.01)] 18 Claims
OG exemplary drawing
 
1. A nanopore array comprising:
a solid-state insulating membrane having a first side and a second side opposite to the first side, the solid-state membrane having a plurality of nanopores, wherein each nanopore of the plurality of nanopores comprises walls and is open to the first side and to the second side, wherein each nanopore is capable of enabling at least a portion of an analyte to pass through each nanopore;
wherein the walls of each nanopore comprise an organic chemical coating comprising a self-assembled mono layer disposed inside the walls of each nanopore;
wherein the organic chemical coating comprises a methoxyethylene glycol-terminated silane monolayer, the organic chemical coating providing at least one surface characteristic of each nanopore without a specific binding between the analyte and each nanopore; and
wherein the at least one surface characteristic includes at least one of a concavity characteristic, a surface charge characteristic, a polarity characteristic, a pH sensitivity characteristic, a hydrophobicity characteristic, and a functionality characteristic, to control the rate of passage of the analyte through each nanopore.