US 12,455,257 B2
Electrochemical aptamer biosensor that detects target material
Hyo Il Jung, Seoul (KR); Gurudatt Nanjanagudu Ganesh, Seoul (KR); Kyung Yeon Lee, Seoul (KR); and Woong Heo, Seoul (KR)
Assigned to UIF (UNIVERSITY INDUSTRY FOUNDATION), YONSEI UNIVERSITY, Seoul (KR)
Filed by UIF (University Industry Foundation), Yonsei University, Seoul (KR)
Filed on Dec. 27, 2022, as Appl. No. 18/146,726.
Claims priority of application No. 10-2022-0004576 (KR), filed on Jan. 12, 2022.
Prior Publication US 2023/0288364 A1, Sep. 14, 2023
Int. Cl. G01N 27/327 (2006.01); B82Y 15/00 (2011.01)
CPC G01N 27/3278 (2013.01) [B82Y 15/00 (2013.01)] 16 Claims
OG exemplary drawing
 
1. An electrochemical aptamer biosensor comprising:
an electrode having conductivity;
a composite graphene oxide layer located on the electrode and including a plurality of graphene oxide plates having a sandwich structure and metal nanoparticles; and
a plurality of target material-specific aptamers immobilized on the composite graphene oxide layer and having nanoparticles,
wherein the composite graphene oxide layer further includes an ionic liquid, and
the graphene oxide plates have the sandwich structure by π-π interaction with the ionic liquid,
wherein the ionic liquid is a 1-ethyl-3-methylimidazolium (EMIM)-based ionic liquid,
wherein the target material is a phthalate-based material,
wherein the electrochemical aptamer biosensor is configured such that a concentration of a target material is measured through an electrochemical signal that is reduced as the target material changes a shape of each of the target material-specific aptamers having nanoparticles,
wherein the electrochemical aptamer biosensor is configured so as to be able to measure a concentration of the target material in a range of 0.14 pg/mL to 7 ng/ml,
wherein a detection limit of the electrochemical aptamer biosensor is 0.042 pg/mL or less.