US 12,455,229 B2
Phasing correction
Robert Langlois, San Diego, CA (US); and Paul Belitz, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Aug. 31, 2021, as Appl. No. 17/446,464.
Application 17/446,464 is a continuation of application No. 15/863,241, filed on Jan. 5, 2018, granted, now 11,150,179.
Claims priority of provisional application 62/443,294, filed on Jan. 6, 2017.
Prior Publication US 2021/0389236 A1, Dec. 16, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/25 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); G01J 3/02 (2006.01); G01N 15/1433 (2024.01); G01N 21/05 (2006.01); G01N 21/64 (2006.01); G06F 5/00 (2006.01); G16B 25/00 (2019.01); G16B 30/00 (2019.01); G16B 30/10 (2019.01); H04N 13/15 (2018.01)
CPC G01N 21/253 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); C12N 15/1068 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); G01J 3/0267 (2013.01); G01N 15/1433 (2024.01); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02); H04N 13/15 (2018.05); G01N 2021/058 (2013.01); G01N 21/6454 (2013.01); G01N 21/6486 (2013.01); G06F 5/00 (2013.01); G06F 2201/805 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of determining corrected color values from image data acquired, during a base calling cycle, by a nucleic acid sequencer comprising an image acquisition system, one or more processors, and memory, the method comprising:
(a) obtaining an image of a substrate comprising a plurality of sites where nucleic acid bases are read, wherein the sites exhibit colors representing nucleic acid base types;
(b) measuring color values of the plurality of sites from the image of the substrate;
(c) storing the color values in a processor buffer of the sequencer's one or more processors;
(d) retrieving phasing-corrected color values of the plurality of sites, wherein the phasing-corrected color values were stored in the sequencer's memory during an immediately preceding base calling cycle; and
(e) determining the corrected color values from
the color values in the processor buffer, and
the phasing corrected values stored during the immediately preceding cycle.