US 12,455,218 B2
Methods and compositions for reversing platelet clumping
Florence Y. Lee, San Diego, CA (US); Renee L Higgins, Santee, CA (US); Jeffrey A. Hawkins, San Marcos, CA (US); and Dena C. Marrinucci, San Diego, CA (US)
Assigned to Truvian Sciences, Inc., San Diego, CA (US)
Appl. No. 17/608,458
Filed by Truvian Sciences, Inc., San Diego, CA (US)
PCT Filed May 8, 2020, PCT No. PCT/US2020/032130
§ 371(c)(1), (2) Date Nov. 2, 2021,
PCT Pub. No. WO2020/227643, PCT Pub. Date Nov. 12, 2020.
Claims priority of provisional application 62/845,807, filed on May 9, 2019.
Prior Publication US 2022/0214253 A1, Jul. 7, 2022
Int. Cl. G01N 1/28 (2006.01); G01N 1/38 (2006.01); G01N 33/86 (2006.01)
CPC G01N 1/38 (2013.01) [G01N 1/286 (2013.01); G01N 33/86 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for dissolution of platelet clumps in a blood sample comprising collecting the blood sample in a heparin-containing container, allowing platelets in the blood sample to clump, and contacting the blood sample in the heparin-containing container with a chelator that reduces reactive Ca2+ from the blood sample thereby reversing platelet clumping, wherein the chelator is present at a concentration of less than about 50% by volume or about 60 mM.