US 12,455,216 B2
Sampling method
Masatsugu Igarashi, Kanagawa (JP); and Takemi Kobayashi, Kanagawa (JP)
Assigned to Terumo Kabushiki Kaisha, (JP)
Filed by Terumo Kabushiki Kaisha, Tokyo (JP)
Filed on Jun. 2, 2023, as Appl. No. 18/205,209.
Application 18/205,209 is a continuation of application No. PCT/JP2022/003050, filed on Jan. 27, 2022.
Claims priority of application No. 2021-014839 (JP), filed on Feb. 2, 2021.
Prior Publication US 2023/0304902 A1, Sep. 28, 2023
Int. Cl. C12M 1/26 (2006.01); G01N 1/20 (2006.01)
CPC G01N 1/2035 (2013.01) 20 Claims
OG exemplary drawing
 
1. A sampling method comprising:
a sampling step including
causing a sample to flow to and through a sampling flow path of a sampling system from a cell culturing device, the sampling system including a biosensor disposed in the sampling flow path and in fluid contact with the sample, and
measuring a concentration of a predetermined component in the sample by the biosensor;
a cleaning step including
causing a cleaning solution to flow from an introduction path of the sampling system into the sampling flow path upstream of the biosensor, the introduction path joining the sampling flow path at a connection portion; and
a returning step including
causing the sample in the sampling flow path to flow into the introduction path upstream of the connection portion
wherein the sampling step is performed twice or more, and the sampling step at second and subsequent times is performed after the cleaning step.