US 12,454,771 B2
Substrates, peptide arrays, and methods
John J. Rajasekaran, Hillsborough, CA (US); Vasanth Jayaraman, San Mateo, CA (US); Tianhao Wang, San Mateo, CA (US); Kang Bei, San Mateo, CA (US); and Hari Krishnan Krishnamurthy, San Mateo, CA (US)
Assigned to Vibrant Holdings LLC, San Carlos, CA (US)
Filed by Vibrant Holdings LLC, San Carlos, CA (US)
Filed on Sep. 19, 2022, as Appl. No. 17/933,465.
Application 17/933,465 is a division of application No. 16/581,730, filed on Sep. 24, 2019, granted, now 11,565,231.
Application 16/581,730 is a division of application No. 14/941,404, filed on Nov. 13, 2015, granted, now 10,486,129, issued on Nov. 26, 2019.
Application 14/941,404 is a division of application No. 14/454,554, filed on Aug. 7, 2014, granted, now 9,216,399, issued on Dec. 22, 2015.
Application 14/454,554 is a continuation of application No. PCT/US2013/025190, filed on Feb. 7, 2013.
Claims priority of provisional application 61/761,347, filed on Feb. 6, 2013.
Claims priority of provisional application 61/726,515, filed on Nov. 14, 2012.
Claims priority of provisional application 61/665,489, filed on Jun. 28, 2012.
Claims priority of provisional application 61/609,003, filed on Mar. 9, 2012.
Claims priority of provisional application 61/608,554, filed on Mar. 8, 2012.
Claims priority of provisional application 61/595,908, filed on Feb. 7, 2012.
Claims priority of provisional application 61/595,988, filed on Feb. 7, 2012.
Prior Publication US 2023/0294063 A1, Sep. 21, 2023
Int. Cl. C40B 50/06 (2006.01); C40B 40/10 (2006.01); C40B 60/14 (2006.01)
CPC C40B 40/10 (2013.01) [C40B 60/14 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method of preparing a substrate for attachment of a coupling reagent, comprising:
obtaining a substrate comprising a planar layer comprising a metal and having an upper surface and a lower surface; and a plurality of pillars operatively coupled to the layer in positionally-defined locations, wherein each pillar has a planar surface extended from the layer, wherein the distance between the surface of each pillar and the upper surface of the layer is between 1,000-5,000 angstroms, wherein the surface of each pillar is parallel to the upper surface of the layer, wherein a linker molecule is attached to the surface of each pillar, wherein the substrate is contacted with a photoactive formulation, and wherein the plurality of pillars are present at a density of greater than 10,000/cm2; and
exposing the substrate to ultraviolet light.