US 12,454,727 B2
Soybean transgenic event MON87751 and methods for detection and use thereof
Kim A. Beazley, Kirkwood, MO (US); Wen C. Burns, Chesterfield, MO (US); Robert H. Cole, II, Florissant, MO (US); Ted C. MacRae, St. Louis, MO (US); John A. Miklos, Des Peres, MO (US); Lisa G. Ruschke, St. Louis, MO (US); Kairong Tian, St. Louis, MO (US); Liping Wei, St. Louis, MO (US); and Kunsheng Wu, Ballwin, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Aug. 11, 2023, as Appl. No. 18/448,493.
Application 16/745,320 is a division of application No. 15/635,041, filed on Jun. 27, 2017, granted, now 10,584,391, issued on Mar. 10, 2020.
Application 15/635,041 is a division of application No. 14/303,042, filed on Jun. 12, 2014, granted, now 9,719,145, issued on Aug. 1, 2017.
Application 18/448,493 is a continuation of application No. 17/568,571, filed on Jan. 4, 2022, granted, now 11,767,568.
Application 17/568,571 is a continuation of application No. 16/745,320, filed on Jan. 16, 2020, granted, now 11,236,399, issued on Feb. 1, 2022.
Claims priority of provisional application 61/834,899, filed on Jun. 14, 2013.
Prior Publication US 2024/0084407 A1, Mar. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); A23D 9/00 (2006.01); A23J 1/14 (2006.01); A23K 10/00 (2016.01); C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)
CPC C12Q 1/6895 (2013.01) [A23D 9/00 (2013.01); A23J 1/14 (2013.01); A23K 10/00 (2016.05); C12N 15/8286 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/158 (2013.01); Y02A 40/146 (2018.01)] 14 Claims
OG exemplary drawing
 
1. A recombinant DNA molecule detectable in a sample containing soybean DNA, wherein the nucleotide sequence of said molecule comprises the polynucleotide sequence of SEQ ID NO: 10 or a complete complement thereof.
 
3. A DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with a junction sequence of soybean event MON87751 DNA in a sample, wherein detecting hybridization of said DNA molecule under said hybridization conditions is diagnostic for soybean event MON87751 DNA in said sample.
 
4. A composition comprising a DNA amplicon comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 18-21, 24, and 26.
 
5. A method of detecting the presence of a DNA segment diagnostic for soybean event MON87751 DNA in a sample, said method comprising:
a) contacting said sample with the DNA probe of claim 3;
b) subjecting said sample and said DNA probe to stringent hybridization conditions; and
c) detecting hybridization of said DNA probe to said sample,
wherein said detecting step is diagnostic for the presence of said soybean event MON87751 DNA in said sample.
 
6. A method of detecting the presence of a DNA segment diagnostic for soybean event MON87751 DNA in a sample, said method comprising:
a) producing a thermal amplification reaction comprising the composition of claim 4 and a pair of DNA molecules comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule that function as DNA primers when used together in an amplification reaction with a sample containing soybean event MON87751 template DNA to produce the DNA amplicon by performing an amplification reaction sufficient to produce the DNA amplicon;
b) detecting the presence of said DNA amplicon wherein said detecting the presence of said amplicon is diagnostic for soybean event MON87751 DNA in said sample; and
c) identifying the sample comprising soybean event MON87751 DNA.