	US 12,454,721 B2
	Template switch-based methods for producing a product nucleic acid
Craig Betts, Mountain View, CA (US); Andrew Alan Farmer, Los Altos, CA (US); and Nathalie Bolduc, Mountain View, CA (US)
Assigned to Takara Bio USA, Inc., San Jose, CA (US)
Filed by Takara Bio USA, Inc., Mountain View, CA (US)
Filed on Apr. 8, 2021, as Appl. No. 17/225,511.
Application 15/194,414 is a division of application No. 14/058,038, filed on Oct. 18, 2013, granted, now 9,410,173, issued on Aug. 9, 2016.
Application 17/225,511 is a continuation of application No. 15/194,414, filed on Jun. 27, 2016, granted, now 11,001,882.
Claims priority of provisional application 61/717,973, filed on Oct. 24, 2012.
Prior Publication US 2021/0222236 A1, Jul. 22, 2021
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6853 (2018.01)

CPC C12Q 1/6853 (2013.01) [C12N 15/1096 (2013.01); C12P 19/34 (2013.01); C12Y 207/07007 (2013.01); C12Y 301/00 (2013.01); C12Q 2521/107 (2013.01); C12Q 2525/191 (2013.01)]

22 Claims

1. A population of double stranded nucleic acids, the population of double stranded nucleic acids comprising:

double stranded nucleic acids that comprise a target template DNA adjacent to a template switch oligonucleotide, wherein the target template DNA and the template switch oligonucleotide are hybridized to a single product nucleic acid, wherein the single product nucleic acid is a single stranded cDNA, wherein the single stranded cDNA comprises a sequence complementary to the template switch oligonucleotide adjacent to a sequence complementary to the target template DNA, and wherein the sequence complementary to the template switch oligonucleotide is at the 3′ end of the single stranded cDNA.