US 12,454,719 B1
Linked ligation
Andrea Marziali, North Vancouver (CA); and Joel Pel, Vancouver (CA)
Assigned to NCAN GENOMICS, INC., Vancouver (CA)
Filed by NCAN Genomics, Inc., Vancouver (CA)
Filed on Jul. 14, 2025, as Appl. No. 19/268,022.
Application 19/268,022 is a continuation of application No. 18/488,255, filed on Oct. 17, 2023.
Application 18/488,255 is a continuation of application No. 17/688,220, filed on Mar. 7, 2022, granted, now 11,879,151, issued on Jan. 23, 2024.
Application 17/688,220 is a continuation of application No. 16/467,870, granted, now 11,268,137, issued on Mar. 8, 2022, previously published as PCT/IB2017/057732, filed on Dec. 7, 2017.
Claims priority of provisional application 62/569,824, filed on Oct. 9, 2017.
Claims priority of provisional application 62/432,277, filed on Dec. 9, 2016.
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6855 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6827 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6855 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); C12Q 2521/501 (2013.01); C12Q 2525/161 (2013.01); C12Q 2525/191 (2013.01); C12Q 2531/113 (2013.01); C12Q 2535/122 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A sequencing adapter construct comprising:
a first adapter portion comprising a first oligonucleotide annealed to a second oligonucleotide to provide a first duplex portion available for a first ligation to a fragment;
a second adapter portion comprising at least a third oligonucleotide providing a second duplex portion available for a second ligation to the fragment; and
a central portion positioned between the first adapter portion and the second adapter portion and within which the second oligonucleotide is linked to a nucleic acid segment that is annealed to the third oligonucleotide over a region of complementarity to form a third duplex portion positioned within the central portion.