	US 12,454,717 B2
	Immune profiling using small volume blood samples
Matthew W. Thomson, Pasadena, CA (US); Tatyana Dobreva, Pasadena, CA (US); David Brown, Pasadena, CA (US); and Jong Hwee Park, Pasadena, CA (US)
Assigned to California Institute of Technology, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Jul. 11, 2023, as Appl. No. 18/350,601.
Application 18/350,601 is a continuation of application No. 17/209,229, filed on Mar. 23, 2021, granted, now 11,739,369.
Claims priority of provisional application 62/993,541, filed on Mar. 23, 2020.
Prior Publication US 2024/0011075 A1, Jan. 11, 2024
Int. Cl. C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01)

CPC C12Q 1/6806 (2013.01) [C12Q 1/6869 (2013.01); C12Q 2600/112 (2013.01)]

20 Claims

1. A method for single cell sequencing comprising:

providing a plurality of capillary blood samples obtained from a plurality of subjects;

isolating immune cells from each of the plurality of samples to obtain isolated immune cells;

pooling the isolated immune cells of the plurality of subjects to obtain pooled immune cells of the plurality of subjects;

performing single cell sequencing on the pooled immune cells of the plurality of subjects to generate single cell sequencing data of the plurality of subjects; and

determining a single cell profile of each of the plurality of subjects using the single cell sequence data of the plurality of subjects and single-nucleotide polymorphisms (SNPs) of the plurality of subjects.