US 12,454,715 B2
Aminoacyl-tRNA synthetase efficiently introducing lysine derivatives
Huiling Liu, Jiangsu (CN); Song Wu, Jiangsu (CN); Zhenshan Zhang, Jiangsu (CN); and Wei Chen, Jiangsu (CN)
Assigned to SUZHOU KUNPENG BIOTECH CO., LTD., Jiangsu (CN)
Appl. No. 17/594,841
Filed by SUZHOU KUNPENG BIOTECH CO., LTD., Jiangsu (CN)
PCT Filed Apr. 30, 2020, PCT No. PCT/CN2020/088506
§ 371(c)(1), (2) Date Nov. 1, 2021,
PCT Pub. No. WO2020/221365, PCT Pub. Date Nov. 5, 2020.
Claims priority of application No. 201910364788.7 (CN), filed on Apr. 30, 2019.
Prior Publication US 2022/0290202 A1, Sep. 15, 2022
Int. Cl. C12P 21/02 (2006.01); C12N 1/20 (2006.01); C12N 9/00 (2006.01); C12N 15/70 (2006.01)
CPC C12P 21/02 (2013.01) [C12N 1/20 (2013.01); C12N 9/93 (2013.01); C12N 15/70 (2013.01); C12Y 601/01006 (2013.01); C12N 2800/101 (2013.01)] 3 Claims
 
1. A mutant protein of aminoacyl-tRNA synthetase, which comprises:
(1) a deletion mutation of amino acid residue at position 102, positions 128-140, and positions 159-179 in an amino acid sequence corresponding to a wild-type aminoacyl-tRNA synthetase; or
(2) a deletion mutation of amino acid residues at position 102, positions 128-140, and positions 159-179 in an amino acid sequence corresponding to a wild-type aminoacyl-tRNA synthetase by replacing positions 1-184 of the wild-type aminoacyl-tRNA synthetase with another segment of amino acids at positions 1-149 of SEQ ID NO: 4; and an amino acid mutation of Y384F corresponding to a wild-type aminoacyl-tRNA synthetase; or
(3) on the basis of (2), further mutations of H29Y, D76G, S89G, N91T, R96K, S121P, N129D, S145P and A148T, as related to SEQ ID NO: 4; or
(4) on the basis of (2), further mutations of L274A, C313S, and F349Y, as related to SEQ ID NO: 4;
Wherein the amino acid sequence of the wild-type aminoacyl-tRNA synthetase is shown in SEQ ID NO: 1 or 2; and
except for the deletion and the mutation, the other amino acids of the mutant protein are the same as the sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2.