US 12,454,708 B2
Use of biological enzyme for preparing orlistat intermediate, and preparation method
Tianshuai Xu, Chongqing (CN); Dayong Gong, Chongqing (CN); Yumei Xiao, Chongqing (CN); Zhanghong Wang, Chongqing (CN); Zhichuan Huang, Chongqing (CN); Shan Huang, Chongqing (CN); Lei Zhang, Chongqing (CN); Xin Gao, Chongqing (CN); and Junwei Shen, Chongqing (CN)
Assigned to ZEIN BIOTECHNOLOGY CO., LTD., Chongqing (CN)
Appl. No. 17/790,787
Filed by ZEIN BIOTECHNOLOGY CO., LTD., Chongqing (CN)
PCT Filed Jan. 6, 2021, PCT No. PCT/CN2021/070495
§ 371(c)(1), (2) Date Jul. 5, 2022,
PCT Pub. No. WO2021/139689, PCT Pub. Date Jul. 15, 2021.
Claims priority of application No. 202010015510.1 (CN), filed on Jan. 7, 2020.
Prior Publication US 2024/0158819 A1, May 16, 2024
Int. Cl. C12P 7/42 (2006.01); C12N 1/21 (2006.01); C12N 9/04 (2006.01); C12N 15/62 (2006.01)
CPC C12P 7/42 (2013.01) [C12N 9/0006 (2013.01); C12N 15/62 (2013.01); C07K 2319/00 (2013.01); C12Y 101/01002 (2013.01); C12Y 101/01047 (2013.01)] 18 Claims
 
1. A composition, comprising:
a biological enzyme; and
a substrate, wherein:
the substrate is a β-carbonyl tetradecanoate which has the structural formula as shown in structural formula I;
the biological enzyme is a ketoreductase comprising the amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2;
a weight ratio of the biological enzyme to the substrate is 1:1.1 to 1:150;
in structural formula I, R is any one of methyl, ethyl, n-propyl or isopropyl; and
structural formula I is:

OG Complex Work Unit Chemistry