US 12,454,703 B2
Controllable transcription
Ludovic Vallier, Cambridgeshire (GB); Mark Kotter, Cambridgeshire (GB); Matthias Pawlowski, Cambridgeshire (GB); Alessandro Bertero, Cambridgeshire (GB); and Daniel Ortmann, Cambridgeshire (GB)
Assigned to Cambridge Enterprise Limited, Cambridgeshire (GB)
Filed by Cambridge Enterprise Limited (GB/GB), Cambridgeshire (GB)
Filed on Nov. 8, 2024, as Appl. No. 18/941,863.
Application 18/941,863 is a division of application No. 18/203,919, filed on May 31, 2023.
Application 18/203,919 is a division of application No. 16/463,829, granted, now 11,697,823, issued on Jul. 11, 2023, previously published as PCT/GB2017/053531, filed on Nov. 24, 2017.
Claims priority of application No. 1619876 (GB), filed on Nov. 24, 2016.
Prior Publication US 2025/0101460 A1, Mar. 27, 2025
Int. Cl. C12N 15/86 (2006.01); A61K 35/545 (2015.01); C12N 5/0735 (2010.01); C12N 5/074 (2010.01); C12N 5/077 (2010.01); C12N 5/079 (2010.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); G01N 33/50 (2006.01)
CPC C12N 15/86 (2013.01) [A61K 35/545 (2013.01); C12N 5/0606 (2013.01); C12N 5/0607 (2013.01); C12N 5/0622 (2013.01); C12N 5/0658 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); G01N 33/5005 (2013.01); C12N 2310/20 (2017.05); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/03 (2013.01); C12N 2750/14143 (2013.01); C12N 2800/80 (2013.01); C12N 2830/003 (2013.01); G01N 2800/00 (2013.01)] 27 Claims
 
1. A cell, comprising: a modified genome comprising an inserted gene encoding a transcriptional regulator protein into a first genetic safe harbour site; and an inserted inducible cassette comprising a transgene operably linked to an inducible promoter into a second genetic safe harbour site, wherein said inducible promoter is regulated by said transcriptional regulator protein, and said first and second sites are different.