US 12,454,698 B2
Transgenic plant with reduced heavy metals and methods for preparation thereof
Hyo Seok Seo, Sejong (KR); Young Gi Lee, Daejeon (KR); Jeong Heon Lee, Daejeon (KR); Kwang Chul Kim, Daejeon (KR); Woong Hyun Na, Daejeon (KR); Kyoung Hwan Oh, Cheongju-si (KR); and Eun Young Jeon, Sejong (KR)
Assigned to KT&G CORPORATION, Daejeon (KR)
Appl. No. 17/642,050
Filed by KT&G CORPORATION, Daejeon (KR)
PCT Filed Aug. 19, 2021, PCT No. PCT/KR2021/011059
§ 371(c)(1), (2) Date Mar. 10, 2022,
PCT Pub. No. WO2022/055144, PCT Pub. Date Mar. 17, 2022.
Claims priority of application No. 10-2020-0114891 (KR), filed on Sep. 8, 2020.
Prior Publication US 2024/0052360 A1, Feb. 15, 2024
Int. Cl. C12N 15/82 (2006.01); C12N 9/10 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/8271 (2013.01) [C12N 9/104 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Y 203/02015 (2013.01); C12N 2310/20 (2017.05)] 5 Claims
 
1. A cell of a Nicotiana species plant having reduced heavy metal absorption in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced by a CRISPR/Cas9 system comprising a single guide RNA(sgRNA) targeting the PCS gene, as compared with a wild-type cell,
wherein the PCS gene is a PCS gene derived from Nicotiana sylvestris (NtPCSs), a PCS gene derived from Nicotiana tomentosiformis (NtPCSt), or a combination thereof (NtPCSst),
wherein the sgRNA targeting the NtPCSs gene consists of the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, the sgRNA targeting the NtPCSt gene consists of the nucleotide sequence of SEQ ID NO: 3 or SEQ ID NO: 4, and the sgRNA targeting the NtPCSs gene and the NtPCSt gene consists of the nucleotide sequence of SEQ ID NO: 5 or SEQ ID NO: 6.