	US 12,454,686 B2
	In vitro cleavage of DNA using argonaute
Nathan Tanner, West Newbury, MA (US); and Eric Hunt, Danvers, MA (US)
Assigned to New England Biolabs, Inc., Ipswich, MA (US)
Filed by New England Biolabs, Inc., Ipswich, MA (US)
Filed on Sep. 7, 2022, as Appl. No. 17/930,079.
Application 17/930,079 is a continuation of application No. 16/018,806, filed on Jun. 26, 2018, granted, now 11,466,264.
Claims priority of provisional application 62/526,019, filed on Jun. 28, 2017.
Prior Publication US 2023/0002754 A1, Jan. 5, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/195 (2006.01); C12N 9/12 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01); C12N 15/90 (2006.01)

CPC C12N 15/102 (2013.01) [C07K 14/195 (2013.01); C12N 9/1252 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 15/90 (2013.01); C12N 2310/14 (2013.01)]

32 Claims

1. A composition comprising:

a bacterial thermostable DNA guided Argonaute protein and

an archaeal thermostable single-stranded DNA binding protein (SSB),

wherein the composition excludes helicase and excludes recombinase.