US 12,454,683 B2
Modified archaeal family B polymerases
Souad Naji, San Diego, CA (US); Carl W. Fuller, New Jersey, NJ (US); Eli N. Glezer, Del Mar, CA (US); and Andrew Spaventa, San Diego, CA (US)
Assigned to Singular Genomics Systems, Inc., San Diego, CA (US)
Filed by Singular Genomics Systems, Inc., San Diego, CA (US)
Filed on Nov. 2, 2023, as Appl. No. 18/500,785.
Application 18/500,785 is a continuation of application No. 17/369,593, filed on Jul. 7, 2021, granted, now 11,845,923.
Application 17/369,593 is a continuation of application No. 16/568,089, filed on Sep. 11, 2019, granted, now 11,136,565, issued on Oct. 5, 2021.
Claims priority of provisional application 62/729,875, filed on Sep. 11, 2018.
Prior Publication US 2024/0101979 A1, Mar. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01); C12P 19/34 (2006.01)
CPC C12N 9/1252 (2013.01) [C12P 19/34 (2013.01); C12Y 207/07007 (2013.01)] 19 Claims
 
1. A polymerase comprising an amino acid sequence that is at least 85% identical to a continuous 500 amino acid sequence within SEQ ID NO: 1; comprising the following amino acids:
an alanine at amino acid position 409 or at a position corresponding to amino acid position 409;
a glycine at amino acid position 410 or at a position corresponding to amino acid position 410;
an alanine at amino acid position 144 or at a position corresponding to amino acid position 144; and
a serine at amino acid position 515 or at a position corresponding to amino acid position 515.