	US 12,454,681 B2
	Erythrocyte removal device, mononuclear cell collector, cell culture device, cell culture system, cell culture method, and mononuclear cell collection method
Koji Tanabe, Palo Alto, CA (US); Ryoji Hiraide, Kyoto (JP); and Kenta Suto, Palo Alto, CA (US)
Assigned to I Peace, Inc., Palo Alto, CA (US)
Appl. No. 17/617,876
Filed by I Peace, Inc., Palo Alto, CA (US); and Koji Tanabe, Palo Alto, CA (US)
PCT Filed Jun. 10, 2020, PCT No. PCT/JP2020/022839 § 371(c)(1), (2) Date Dec. 9, 2021, PCT Pub. No. WO2020/250927, PCT Pub. Date Dec. 17, 2020.
Claims priority of provisional application 62/859,307, filed on Jun. 10, 2019.
Prior Publication US 2022/0306993 A1, Sep. 29, 2022
Int. Cl. C12N 5/074 (2010.01); C12N 5/00 (2006.01)

CPC C12N 5/0696 (2013.01) [C12N 5/0087 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/606 (2013.01); C12N 2506/11 (2013.01)]

12 Claims

1. A cell culture method comprising:

introducing an induction factor into mononuclear cells in a cell culture vessel; and

culturing the mononuclear cells into which the induction factor has been introduced in the same cell culture vessel until iPS cells are induced from the mononuclear cells into which the induction factor has been introduced,

wherein the cell culture vessel is closed during the introducing and the culturing, and

gas in the cell culture vessel is not exchanged during the culturing.