	US 12,454,675 B2
	Method of increasing protein yield in mammalian cells in serum-free culture medium
Say Kong Ng, Singapore (SG); Sow Zong Dawn Leong, Singapore (SG); Gek Ling Janice Tan, Singapore (SG); and Kah Hui Brian Teo, Singapore (SG)
Assigned to AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH, Singapore (SG)
Filed by AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH, Singapore (SG)
Filed on Nov. 23, 2021, as Appl. No. 17/534,399.
Application 17/534,399 is a continuation of application No. 16/079,070, abandoned, previously published as PCT/SG2017/050079, filed on Feb. 22, 2017.
Claims priority of application No. 10201601284Y (SG), filed on Feb. 22, 2016.
Prior Publication US 2022/0154137 A1, May 19, 2022
Int. Cl. C12N 5/00 (2006.01)

CPC C12N 5/0037 (2013.01) [C12N 5/0031 (2013.01); C12N 2500/34 (2013.01); C12N 2500/90 (2013.01)]

15 Claims

1. A method of extending cell culture viability of a mammalian cell, comprising: growing and culturing the mammalian cell in a serum-free cell culture medium, wherein the culture medium comprises maltose and glucose as sole carbohydrate sources, wherein the maltose is present at a concentration of between 0.5 g/litre to 40 g/litre, wherein the maltose and glucose results in extended cell culture viability, and wherein maximum viable cell density is increased by at least 4% as compared with glucose as the sole carbohydrate source.