US 12,454,667 B2
Methods of cell culture for adoptive cell therapy
Juan F. Vera, Bellaire, TX (US); Cliona M. Rooney, Bellaire, TX (US); Ann M. Leen, Bellaire, TX (US); and John R. Wilson, New Brighton, MN (US)
Assigned to Wilson Wolf Manufacturing, LLC, New Brighton, MN (US); and Baylor College of Medicine, Houston, TX (US)
Filed by Wilson Wolf Manufacturing, New Brighton, MN (US); and Baylor College of Medicine, Houston, TX (US)
Filed on Jun. 3, 2024, as Appl. No. 18/732,410.
Application 16/696,557 is a division of application No. 15/395,662, filed on Dec. 30, 2016, granted, now 10,533,156, issued on Jan. 14, 2020.
Application 14/579,373 is a division of application No. 13/475,700, filed on May 18, 2012, granted, now 8,956,860, issued on Feb. 17, 2015.
Application 18/732,410 is a continuation of application No. 18/511,208, filed on Nov. 16, 2023, granted, now 11,999,969.
Application 18/511,208 is a continuation of application No. 17/688,477, filed on Mar. 7, 2022, granted, now 11,821,002, issued on Nov. 21, 2023.
Application 17/688,477 is a continuation of application No. 16/696,557, filed on Nov. 26, 2019, granted, now 11,268,066, issued on Mar. 8, 2022.
Application 15/395,662 is a continuation of application No. 14/579,373, filed on Dec. 22, 2014, granted, now 9,567,565, issued on Feb. 14, 2017.
Application 13/475,700 is a continuation in part of application No. 12/963,597, filed on Dec. 8, 2010, granted, now 8,809,050, issued on Aug. 19, 2014.
Claims priority of provisional application 61/267,761, filed on Dec. 8, 2009.
Prior Publication US 2024/0318137 A1, Sep. 26, 2024
Int. Cl. C12M 1/04 (2006.01); A61K 39/00 (2006.01); C12N 5/0783 (2010.01)
CPC C12M 23/24 (2013.01) [A61K 39/0011 (2013.01); C12N 5/0638 (2013.01); A61K 2039/5156 (2013.01); A61K 2039/5158 (2013.01); C12N 2501/2302 (2013.01); C12N 2502/11 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A static culture method for expanding a population of cells within a static gas permeable cell culture and cell recovery device having a growth surface comprised of liquid impermeable gas permeable material, at least a portion of said liquid impermeable gas permeable material in contact with ambient gas, an internal volume bounded at least in part by said growth surface and an opposing upper surface,
a vent,
a medium removal conduit including a medium removal opening,
a cell removal conduit including a cell removal opening,
and when the device is oriented in the static cell culture position, the growth surface is in a horizontal position and is located at least in part below said opposing upper confine, the distance from said growth surface to said cell removal opening is less than the distance from said growth surface to said medium removal opening, and the maximum distance from the upper confine to the growth surface is the maximum potential medium height of the internal volume and the method comprising:
adding a quantity of T cells and medium into the static gas permeable cell culture device,
allowing static cell culture to continue without feeding for a period of time,
determining the quantity of cells without counting cells by measuring the lactate and/or glucose in the medium.