	US 12,454,567 B2
	Large scale purification of antibodies
Guy Patchornik, Kiryat-Ono (IL)
Assigned to Ariel Scientific Innovations Ltd., Ariel (IL)
Appl. No. 18/291,954
Filed by Ariel Scientific Innovations Ltd., Ariel (IL)
PCT Filed Jul. 27, 2022, PCT No. PCT/IL2022/050818 § 371(c)(1), (2) Date Jan. 25, 2024, PCT Pub. No. WO2023/007497, PCT Pub. Date Feb. 2, 2023.
Claims priority of provisional application 63/225,994, filed on Jul. 27, 2021.
Prior Publication US 2024/0262890 A1, Aug. 8, 2024
Int. Cl. C07K 16/06 (2006.01); C07K 1/32 (2006.01)

CPC C07K 16/065 (2013.01) [C07K 1/32 (2013.01)]

12 Claims

1. A method of isolating an antibody, the method comprising contacting an aggregate comprising a hydrophobic chelator, at least one non-ionic detergent, metal ions and an amino acid with a liquid sample comprising the antibody under conditions that allow partitioning of the antibody into said aggregate, wherein:

said hydrophobic chelator is bathophenanthroline;

said non-ionic detergent being a polysorbate, a polyoxyethylene oleyl ether or octylphenol ethoxylate;

said metal ions are iron ions; and

said amino acid is selected from the group consisting of Phenylalanine (Phe), Tyrosine (Tyr), Tryptophan (Trp), Isoleucine (Ile) and Valine (Val),

thereby isolating the antibody.