	US 12,454,566 B2
	Construction of engineering bacteria for high expression of recombinant human serum albumin
Guodong Chang, Beijing (CN); Xin Dou, Beijing (CN); Xiaobo Xiong, Beijing (CN); and Wenchao Wang, Beijing (CN)
Assigned to Shenzhen Protgen Ltd., Shenzhen (CN)
Appl. No. 16/099,159
Filed by Shenzhen Protgen Ltd., Shenzhen (CN)
PCT Filed May 4, 2017, PCT No. PCT/CN2017/083079 § 371(c)(1), (2) Date Nov. 5, 2018, PCT Pub. No. WO2017/190671, PCT Pub. Date Nov. 9, 2017.
Claims priority of application No. 201610289898.8 (CN), filed on May 4, 2016.
Prior Publication US 2019/0330707 A1, Oct. 31, 2019
Int. Cl. C07K 14/765 (2006.01); C12N 9/02 (2006.01); C12N 15/81 (2006.01); C12R 1/84 (2006.01); C07K 14/395 (2006.01); C12N 1/16 (2006.01)

CPC C07K 14/765 (2013.01) [C12N 9/0051 (2013.01); C12N 15/81 (2013.01); C07K 14/395 (2013.01); C12N 1/165 (2021.05); C12R 2001/84 (2021.05); C12Y 502/01008 (2013.01); C12Y 503/04001 (2013.01)]

12 Claims

1. A method for highly expressing recombinant human serum albumin (rHSA) in a yeast host cell, comprising co-expressing a human serum albumin gene, a gene encoding protein disulfide isomerase (PDI), and a gene encoding transcriptional activator HAC1, wherein the yeast host cell is Pichia pastoris.