US 12,454,541 B2
Reagent for measuring oxygen concentration in cell and tissue
Toshitada Yoshihara, Gunma (JP); Tatsuya Hirose, Gunma (JP); and Seiji Tobita, Gunma (JP)
Assigned to NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY, Gunma (JP)
Appl. No. 17/638,424
Filed by NATIONAL UNIVERSITY CORPORATION GUNMA UNIVERSITY, Gunma (JP)
PCT Filed Aug. 25, 2020, PCT No. PCT/JP2020/032028
§ 371(c)(1), (2) Date Feb. 25, 2022,
PCT Pub. No. WO2021/039789, PCT Pub. Date Mar. 4, 2021.
Claims priority of application No. 2019-153754 (JP), filed on Aug. 26, 2019.
Prior Publication US 2022/0306665 A1, Sep. 29, 2022
Int. Cl. C07F 15/00 (2006.01); A61K 49/00 (2006.01); G01N 21/64 (2006.01)
CPC C07F 15/0033 (2013.01) [A61K 49/0015 (2013.01); G01N 21/64 (2013.01)] 10 Claims
 
1. A method for measuring oxygen concentration, the method comprising administering a compound represented by Formula (I) or (II) to a subject, monitoring a phosphorescence signal of the administered compound, and calculating oxygen concentration from the monitored phosphorescence intensity:

OG Complex Work Unit Chemistry
wherein
in Formula (II), R1 and R2 each independently represent hydrogen or a C1-C6 hydrocarbon group;
in Formula (I), R1 and R2 each independently represent a C2-C6 hydrocarbon group; and
X− represents a counter anion.