US 12,453,745 B2
Method for isolation and purification of microvesicles from cell culture supernatants and biological fluids
Evangelos V. Badiavas, Coral Gables, FL (US); Arsalan Q. Shabbir, Miami, FL (US); and Stephen C. Davis, El Portal, FL (US)
Assigned to UNIVERSITY OF MIAMI, Miami, FL (US)
Filed by UNIVERSITY OF MIAMI, Miami, FL (US)
Filed on Jun. 9, 2023, as Appl. No. 18/332,063.
Application 18/332,063 is a continuation of application No. 16/668,851, filed on Oct. 30, 2019, granted, now 11,730,768.
Application 16/668,851 is a continuation of application No. 14/775,382, granted, now 10,500,231, issued on Sep. 11, 2015, previously published as PCT/US2014/024629, filed on Mar. 12, 2014.
Claims priority of provisional application 61/778,591, filed on Mar. 13, 2013.
Prior Publication US 2023/0364150 A1, Nov. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/28 (2015.01); A61K 8/98 (2006.01); A61K 35/12 (2015.01); A61K 35/16 (2015.01); A61K 35/22 (2015.01); A61Q 19/00 (2006.01); A61Q 19/08 (2006.01)
CPC A61K 35/28 (2013.01) [A61K 8/981 (2013.01); A61K 8/983 (2013.01); A61K 35/12 (2013.01); A61K 35/16 (2013.01); A61K 35/22 (2013.01); A61Q 19/00 (2013.01); A61Q 19/08 (2013.01); A61K 2800/74 (2013.01); A61K 2800/86 (2013.01)] 24 Claims
 
1. A topical composition for treating wounds comprising an aqueous concentrate of a heterogeneous population of purified, intact microvesicles,
wherein the intact microvesicles range in size from 2 nm to 5000 nm,
wherein the intact microvesicles have smooth and uncorrugated borders and appear intact when examined by electron microscopy, and
wherein the intact microvesicles enhance tissue regeneration, promote wound healing, enhance nerve growth, remodel scarred tissue, modulate immune reactions, alter neoplastic cell growth, and/or decrease abscess formation of a tissue when administered to a patient.