US 12,453,696 B2
Active principle comprising a particular extract of Punica granatum and uses for preventing and/or treating acne
Jean-Jacques Paufique, Objat (FR)
Assigned to SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE, Objat (FR)
Appl. No. 17/919,380
Filed by SOCIETE INDUSTRIELLE LIMOUSINE D'APPLICATION BIOLOGIQUE, Objat (FR)
PCT Filed Apr. 16, 2021, PCT No. PCT/EP2021/059897
§ 371(c)(1), (2) Date Nov. 30, 2022,
PCT Pub. No. WO2021/209593, PCT Pub. Date Oct. 21, 2021.
Claims priority of application No. FR2003896 (FR), filed on Apr. 17, 2020.
Prior Publication US 2023/0092216 A1, Mar. 23, 2023
Int. Cl. A61K 8/9789 (2017.01); A61Q 17/00 (2006.01); A61Q 19/00 (2006.01)
CPC A61K 8/9789 (2017.08) [A61Q 17/00 (2013.01); A61Q 19/008 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for obtaining an active ingredient, comprising at least one hydrolysate of the rind of immature fruits of Punica granata, wherein the method comprises at least one aqueous extraction and enzymatic hydrolysis, the method comprises at least the following steps:
a. solubilization of powder of the rind of immature Punica granatum fruit in water in a proportion of at least 50 g/l,
b. enzymatic hydrolysis with the aid of a carbohydrase,
c. separation of soluble and insoluble phases,
d. recovery of the soluble phase and thermal enzymatic inactivation, and
e. purification of polyphenolic compounds.