US 12,453,364 B2
Method for producing a polysaccharide
Kazuhisa Sekimizu, Tokyo (JP); and Makoto Urai, Tokyo (JP)
Assigned to IMAGINE GLOBAL CARE CORPORATION, Tokyo (JP)
Filed by IMAGINE GLOBAL CARE CORPORATION, Tokyo (JP)
Filed on Dec. 3, 2021, as Appl. No. 17/457,534.
Application 17/457,534 is a continuation of application No. 16/309,098, granted, now 11,213,058, previously published as PCT/JP2017/029237, filed on Aug. 4, 2017.
Claims priority of application No. 2016-156274 (JP), filed on Aug. 9, 2016.
Prior Publication US 2022/0160012 A1, May 26, 2022
Int. Cl. A23L 33/125 (2016.01); A23L 19/00 (2016.01)
CPC A23L 33/125 (2016.08) [A23L 19/00 (2016.08)] 2 Claims
OG exemplary drawing
 
1. A method for producing a polysaccharide, comprising:
extracting broccoli with hot water to obtain an extract;
subjecting the extract to ethanol precipitation;
subjecting obtained precipitates to dialysis and freeze drying to obtain a freeze-dried product;
subjecting the freeze-dried product to column chromatography;
collecting peak fractions that give C value (contraction value) of 0.15 or more based on Silkworm Muscle Contraction Assay as purified fractions containing eluted sugars;
subjecting the peak fractions to dialysis and freeze drying;
hydrolyzing the freeze-dried peak fractions with oxalic acid to obtain a hydrolyzed product; and
separating the polysaccharide from the hydrolyzed product,
wherein the polysaccharide has innate immune stimulating activity, and comprises: 25 to 45 parts by mole of galacturonic acid, 20 to 50 parts by mole of galactose, 1 to 7 parts by mole of glucose, 0 to 10 parts by mole of arabinose, 0 to 6 parts by mole of xylose, and 4 to 14 parts by mole of rhamnose as constituent sugars; and a polygalacturonic acid chain having α-1,4-linked galacturonic acid as a main chain.