US 12,453,360 B2
Acetic acid bacteria-containing composition
Wakana Yamanaka, Aichi (JP)
Assigned to Mizkan Holdings Co., Ltd., Aichi (JP)
Filed by Mizkan Holdings Co., Ltd., Aichi (JP)
Filed on Apr. 18, 2022, as Appl. No. 17/723,123.
Application 17/723,123 is a continuation in part of application No. PCT/JP2019/041173, filed on Oct. 18, 2019.
Prior Publication US 2022/0240549 A1, Aug. 4, 2022
Int. Cl. A23L 27/00 (2016.01); A23L 2/52 (2006.01); A23L 33/135 (2016.01)
CPC A23L 2/52 (2013.01) [A23L 33/135 (2016.08); A23V 2002/00 (2013.01); A23V 2200/16 (2013.01)] 15 Claims
 
1. A method for producing an acetic acid bacteria-containing composition,
the method comprising (i) culturing an acetic acid bacteria, (ii) collecting the acetic acid bacteria after step (i), and (iii) drying the acetic acid bacteria after step (ii), wherein:
the acetic acid bacteria is Acetobacter malorum;
step (i) further comprises mixing the acetic acid bacteria with nonanal and methylheptenone so that the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone), as determined by solid-phase microextraction gas chromatography-mass spectrometry, is 1:10 to 100:1; and
in step (i), the acetic acid bacteria comprises acetic acid bacteria, dimethyl trisulfide, and 2-tridecanone so that the peak area ratio of nonanal to methylheptenone (nonanal:methylheptenone) is 1:10 to 100:1, the peak area ratio of nonanal to dimethyl trisulfide (nonanal: dimethyl trisulfide) is 1:100 to 50:1, and the peak area ratio of nonanal to 2-tridecanone (nonanal:2-tridecanone) is 1:100 to 100:1.