US 12,453,346 B2
Stabilization of whole blood at room temperature
Waltraud Ankenbauer, Mannheim (DE); Ulrike Dietrich-Veenstra, Iffeldorf (DE); Renate Kolb, Iffeldorf (DE); Yvonne Maerz, Murnau (DE); and Stephanie Wessner, Farchant (DE)
Assigned to Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed by Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed on Oct. 2, 2020, as Appl. No. 17/061,711.
Application 17/061,711 is a continuation of application No. 15/221,791, filed on Jul. 28, 2016, abandoned.
Application 15/221,791 is a continuation of application No. PCT/EP2015/051658, filed on Jan. 28, 2015.
Claims priority of application No. 14153199 (EP), filed on Jan. 30, 2014.
Prior Publication US 2021/0015092 A1, Jan. 21, 2021
Int. Cl. A01N 1/02 (2006.01); A01N 1/126 (2025.01)
CPC A01N 1/126 (2025.01) 10 Claims
OG exemplary drawing
 
1. A method for stabilizing intact nucleated cells of a whole-blood sample ex-vivo, the method comprising the steps of
(a) providing the whole blood sample ex-vivo;
(b) contacting and mixing the sample of step (a) with an aqueous solution comprising 11.1 mM-12.3 mM trisodium citrate, 2 mM-2.2 mM citric acid, 2 mM-2.2 mM NaH2PO4, 19.1 mM-21.1 mM glucose, 0.23 mM-0.25 mM adenine, 0.24 mM-0.26 mM AEBSF, (4-(2-Aminoethyl)benzenesulfonyl fluoride) 990 nM-1.1 μM SS-31 and 990 μM-1.1 mM acetylsalicylic acid, wherein the solution is enclosed in a blood drawing container;
(c) incubating the mixture obtained in step (b),
thereby stabilizing intact nucleated cells of the ex-vivo whole-blood sample.