US 12,123,042 B2
Production of steviol glycosides in recombinant hosts
Helene Lunde Robertsen, Horsholm (DK); Iben Nordmark Andersen, Vedbaek (DK); Adam Matthew Takos, Valby (DK); Swee Chuang Lim Hallwyl, Vallensbaek Strand (DK); Francesca Ambri, Hillerod (DK); Manuel Quiros Asensio, Soborg (DK); Michael Dalgaard Mikkelsen, Vaerlose (DK); Jens Houghton-Larsen, Birkerod (DK); Veronique Douchin, Birkerod (DK); Jane Dannow Dyekjaer, Frederiksberg (DK); Simon Carlsen, Copenhagen (DK); Nina Nicoline Rasmussen, Hvidovre (DK); and Esben Halkjaer Hansen, Frederiksberg (DK)
Assigned to Danstar Ferment AG, Schweiz (CH)
Filed by EVOLVA SA, Reinach (CH)
Filed on Aug. 30, 2022, as Appl. No. 17/899,069.
Application 16/801,200 is a division of application No. 15/506,196, granted, now 10,612,064, previously published as PCT/EP2015/070620, filed on Sep. 5, 2015.
Application 17/899,069 is a continuation of application No. 16/801,200, filed on Feb. 26, 2020, granted, now 11,466,302.
Claims priority of provisional application 62/148,585, filed on Apr. 16, 2015.
Claims priority of provisional application 62/117,396, filed on Feb. 17, 2015.
Claims priority of provisional application 62/103,547, filed on Jan. 14, 2015.
Claims priority of provisional application 62/048,178, filed on Sep. 9, 2014.
Prior Publication US 2023/0212630 A1, Jul. 6, 2023
Int. Cl. C12P 19/56 (2006.01); A23L 27/30 (2016.01); C07H 15/256 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/52 (2006.01)
CPC C12P 19/56 (2013.01) [A23L 27/36 (2016.08); C07H 15/256 (2013.01); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12Y 106/02004 (2013.01); C12Y 114/13078 (2013.01); C12Y 114/13079 (2013.01); C12Y 205/01029 (2013.01); C12Y 402/03019 (2013.01); C12Y 505/01013 (2013.01); A23V 2002/00 (2013.01); C07K 2319/00 (2013.01)] 13 Claims
 
1. A recombinant host, comprising:
(a) a gene encoding a cytochrome P450 reductase (CPR) polypeptide;
wherein the CPR polypeptide comprises a CPR polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:98; and
(b) a gene encoding an ent-kaurenoic acid hydroxylase (KAH) polypeptide;
wherein the KAH polypeptide comprises a KAH polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:82;
wherein at least one of the genes is a recombinant gene;
wherein the recombinant host produces a steviol or a steviol glycoside precursor; and
wherein the recombinant host cell is a fungal cell.