	US 12,123,039 B2
	Preparation of thermophilic beta-glucosidase and application thereof
Jing Wu, Wuxi (CN); Wei Xia, Wuxi (CN); Xinghao Xu, Wuxi (CN); and Yan Huang, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Nov. 8, 2021, as Appl. No. 17/520,811.
Application 17/520,811 is a continuation of application No. PCT/CN2020/102671, filed on Jul. 17, 2020.
Claims priority of application No. 202010342426.0 (CN), filed on Apr. 27, 2020.
Prior Publication US 2022/0056496 A1, Feb. 24, 2022
Int. Cl. C12P 19/14 (2006.01); C12N 9/42 (2006.01)

CPC C12P 19/14 (2013.01) [C12N 9/2445 (2013.01); C12Y 302/01021 (2013.01)]

8 Claims

1. A method for producing gentioligosaccharide, comprising producing gentioligosaccharide with glucose or a combination of glucose and cellobiose as a substrate using β-glucosidase expressed by a recombinant bacteria carrying a gene with the nucleotide sequence as set forth in SEQ ID NO:1.