	US 12,123,031 B2
	Regulation of CRISPR-associated rossman fold (CARF) domain containing proteins by oligoadenylates
Virginijus Šikšnys, Vilnius (LT); Migle Kazlauskiene, Vilnius (LT); Georgij Kostiuk, Vilnius (LT); and Gintautas Tamulaitis, Vilnius (LT)
Assigned to VILNIUS UNIVERSITY, Vilnius (LT)
Appl. No. 16/618,238
Filed by VILNIUS UNIVERSITY, Vilnius (LT)
PCT Filed May 31, 2018, PCT No. PCT/IB2018/053906 § 371(c)(1), (2) Date Nov. 29, 2019, PCT Pub. No. WO2018/220583, PCT Pub. Date Dec. 6, 2018.
Claims priority of provisional application 62/512,868, filed on May 31, 2017.
Prior Publication US 2021/0130799 A1, May 6, 2021
Int. Cl. C12N 9/22 (2006.01); C12N 9/12 (2006.01); C12P 19/34 (2006.01); C12Q 1/6811 (2018.01)

CPC C12N 9/22 (2013.01) [C12N 9/1241 (2013.01); C12P 19/34 (2013.01); C12Q 1/6811 (2013.01); C12Y 207/07 (2013.01); C12N 2310/20 (2017.05)]

8 Claims

1. A method for activation and/or regulation of a protein containing a CRISPR-Associated Rossmann Fold (CARF) domain using a cyclic oligoadenylate, selected from the group consisting of c(AMP)3, c(AMP)4, c(AMP)6 and c(dAMP)3, or a linear oligoadenylate, selected from the group consisting of tetraadenylate, tetraadenylate phosphate, tetraadenylate triphosphate, hexaadenylate, hexaadenylate phosphate, hexaadenylate triphosphate and any combinations thereof.