	US 12,123,017 B2
	Composition and method for cultivation, expansion, preservation and/or cell pretreatment
Rafael Tapia Limonchi, Santiago (CL); Maroun Khoury, Santiago (CL); and Daniel Meza Rojas, Santiago (CL)
Assigned to REGENERO S.A., Santiago (CL)
Appl. No. 16/767,383
Filed by REGENERO S.A., Santiago (CL)
PCT Filed Nov. 27, 2017, PCT No. PCT/CL2017/050070 § 371(c)(1), (2) Date May 27, 2020, PCT Pub. No. WO2019/100175, PCT Pub. Date May 31, 2019.
Prior Publication US 2020/0385671 A1, Dec. 10, 2020
Int. Cl. C12N 5/00 (2006.01); C12N 5/073 (2010.01); C12N 5/074 (2010.01); C12N 5/0775 (2010.01); C12N 5/0783 (2010.01); C12N 5/0784 (2010.01)

CPC C12N 5/0043 (2013.01) [C12N 5/0037 (2013.01); C12N 5/0056 (2013.01); C12N 5/0603 (2013.01); C12N 5/0637 (2013.01); C12N 5/0639 (2013.01); C12N 5/0662 (2013.01); C12N 5/0696 (2013.01); C12N 2500/22 (2013.01); C12N 2500/30 (2013.01)]

16 Claims

1. A composition for the culture, expansion, or preservation of cells, wherein the composition is a medium, supplement of medium, or solution comprising:

pterostilbene at a concentration between 10 μM to 100 μM,

basic fibroblast growth factor (bFGF) at a concentration between 1 μg/L to about 75 μg/L, and

transforming growth factor beta (TGF-β) at a concentration between 0.5 μg/L to about 5 μg/L,

wherein the composition is serum free.