US 12,122,994 B2
Methods for fermentative production of massoia lactone
Lianghui Ji, Singapore (SG); and Si Te Ngoh, Singapore (SG)
Assigned to TEMASEK LIFE SCIENCES LABORATORY LIMITED, Singapore (SG)
Filed by TEMASEK LIFE SCIENCES LABORATORY LIMITED, Singapore (SG)
Filed on Mar. 20, 2023, as Appl. No. 18/186,257.
Application 18/186,257 is a division of application No. 16/712,672, filed on Dec. 12, 2019, granted, now 11,639,492.
Application 16/712,672 is a division of application No. 15/750,584, abandoned, previously published as PCT/SG2016/050395, filed on Aug. 16, 2016.
Claims priority of provisional application 62/205,996, filed on Aug. 17, 2015.
Prior Publication US 2023/0365915 A1, Nov. 16, 2023
Int. Cl. C12N 1/14 (2006.01); C12P 17/06 (2006.01); C12R 1/645 (2006.01)
CPC C12N 1/14 (2013.01) [C12N 1/145 (2021.05); C12P 17/06 (2013.01); C12N 2500/22 (2013.01); C12N 2500/24 (2013.01); C12N 2500/42 (2013.01); C12R 2001/645 (2021.05)] 20 Claims
 
1. A culture for the fermentative production of Massoia lactone comprising an Aureobasidium melanogenum species in a culture medium, wherein the Aureobasidium melanogenum species expresses no functional Aureobasidin A synthase gene mRNA when cultured, and
wherein the culture medium comprises:
10.0 g/l to 15 g/l KH2PO4, 0.5 g/l to 2.0 g/l Na2HPO4, 3.5 g/l to 6.5 g/l (NH4)2SO4, 1.0 g/l to 4.0 g/12.5 g/l MgSO4·7H2O and 0.10 g/l to 0.40 g/l CaCl2·2H2O;
at least two trace elements selected from the group consisting of Fe2+, Cu2+, Zn2+ and MoO42−, wherein each trace element present in the culture medium is present in an amount from 0.1 μM to 1.0 mM;
1.5 g/l to 2.5 g/l urea; and
a carbon source selected from the group consisting of glucose, mannose, xylose and mixtures thereof, wherein the carbon source present in the culture medium from 4% to 12%,
wherein the culture medium has a pH from 5.5 to 6.5.