US 12,122,990 B2
Culture container base material, culture container, and production method of culture container base material
Yosuke Matsuoka, Kanagawa (JP); Satoshi Tanaka, Kanagawa (JP); Takahiko Totani, Kanagawa (JP); and Takaharu Nishiyama, Kanagawa (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed on Nov. 3, 2020, as Appl. No. 17/087,913.
Application 17/087,913 is a continuation of application No. PCT/JP2019/017580, filed on Apr. 25, 2019.
Claims priority of application No. 2018-088954 (JP), filed on May 3, 2018.
Prior Publication US 2021/0115376 A1, Apr. 22, 2021
Int. Cl. C12M 1/12 (2006.01); C12N 1/00 (2006.01)
CPC C12M 25/00 (2013.01) [C12N 1/00 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01); C12N 2535/00 (2013.01)] 8 Claims
 
1. A culture container base material for culturing adherent cells comprising:
a polyolefin material;
a surface, wherein at least a part of the surface of the culture container base material is subjected to a surface treatment,
wherein:
the surface treatment comprises a hydrophilization treatment and a modification treatment,
a static water contact angle of the at least a part of the surface of the culture container base material that is subjected to the surface treatment comprising the hydrophilization treatment and the modification treatment is greater than 80°,
a receding contact angle of the at least a part of the surface of the culture container base material that is subjected to the surface treatment comprising the hydrophilization treatment and the modification treatment is less than 53°,
and
the modification treatment is performed at a temperature in a range of from 45° C. to 55° C.