US 12,122,840 B2
Method of modifying isoelectric point of antibody via amino acid substitution in CDR
Tomoyuki Igawa, Shizuoka (JP); Hiroyuki Tsunoda, Shizuoka (JP); Tatsuhiko Tachibana, Shizuoka (JP); and Taichi Kuramochi, Shizuoka (JP)
Assigned to Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed by Chugai Seiyaku Kabushiki Kaisha, Tokyo (JP)
Filed on Jan. 29, 2024, as Appl. No. 18/425,859.
Application 18/425,859 is a division of application No. 17/578,524, filed on Jan. 19, 2022.
Application 17/578,524 is a division of application No. 15/725,692, filed on Oct. 5, 2017, granted, now 11,248,053, issued on Feb. 15, 2022.
Application 15/725,692 is a continuation of application No. 14/741,786, filed on Jun. 17, 2015, granted, now 9,828,429, issued on Nov. 28, 2017.
Application 14/741,786 is a continuation of application No. 12/679,922, granted, now 9,096,651, issued on Aug. 4, 2015, previously published as PCT/JP2008/067534, filed on Sep. 26, 2008.
Claims priority of application No. 2007-250165 (JP), filed on Sep. 26, 2007; and application No. 2007-256063 (JP), filed on Sep. 28, 2007.
Prior Publication US 2024/0190976 A1, Jun. 13, 2024
Int. Cl. C07K 16/28 (2006.01); A61K 39/395 (2006.01); C07K 16/00 (2006.01); G01N 33/68 (2006.01)
CPC C07K 16/2866 (2013.01) [A61K 39/39591 (2013.01); C07K 16/00 (2013.01); C07K 16/28 (2013.01); G01N 33/6854 (2013.01); C07K 2317/24 (2013.01); C07K 2317/565 (2013.01); C07K 2317/732 (2013.01)] 24 Claims
 
1. A multispecific antibody comprising a first arm comprising a first heavy chain variable region (VH) and a first light chain variable region (VL) and a second arm comprising a second VH and a second VL, wherein:
the amino acid residue at Kabat numbering position 55 in the first VL is glutamic acid or aspartic acid,
the amino acid residue at Kabat numbering position 55 in the second VL has no charge or has a positive charge; and
the isoelectric point of the first arm is different from the isoelectric point of the second arm.