US 12,122,820 B2
Method of redirecting t cells to treat HIV infection
James L. Riley, Downingtown, PA (US); Rachel Leibman, Philadelphia, PA (US); Aimee S. Payne, Merion Station, PA (US); Christoph T. Ellebrecht, Philadelphia, PA (US); and Michael C. Milone, Cherry Hill, NJ (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US)
Filed on Aug. 7, 2020, as Appl. No. 16/988,415.
Application 16/988,415 is a continuation of application No. 15/761,563, granted, now 10,738,099, previously published as PCT/US2016/053097, filed on Sep. 22, 2016.
Claims priority of provisional application 62/222,132, filed on Sep. 22, 2015.
Claims priority of provisional application 62/253,790, filed on Nov. 11, 2015.
Prior Publication US 2020/0362012 A1, Nov. 19, 2020
Int. Cl. C07H 21/04 (2006.01); A61K 35/17 (2015.01); A61K 38/17 (2006.01); A61K 39/42 (2006.01); A61K 45/06 (2006.01); A61K 48/00 (2006.01); A61P 31/18 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C07K 14/73 (2006.01); C07K 16/10 (2006.01); C12N 15/63 (2006.01); A01K 67/00 (2006.01); A61K 39/00 (2006.01)
CPC C07K 14/70514 (2013.01) [A61K 35/17 (2013.01); A61K 38/1774 (2013.01); A61K 39/42 (2013.01); A61K 45/06 (2013.01); A61P 31/18 (2018.01); C07K 14/7051 (2013.01); C07K 14/70517 (2013.01); C07K 14/70521 (2013.01); C07K 14/70578 (2013.01); C07K 16/1045 (2013.01); A61K 2039/505 (2013.01); A61K 2039/5158 (2013.01); A61K 2039/572 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/622 (2013.01); C07K 2317/76 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/03 (2013.01); C07K 2319/30 (2013.01); C07K 2319/33 (2013.01)] 11 Claims
 
1. A vector comprising a nucleic acid molecule comprising a coding sequence encoding a membrane-bound chimeric receptor comprising a CD4 extracellular domain, a transmembrane domain, and a signaling domain comprising a CD28 costimulatory signaling region, wherein the CD4 extracellular domain is capable of recognizing and binding a HIV infected cell, wherein the nucleic acid molecule further comprises an EF1α promoter operably linked to the coding sequence, wherein the CD4 extracellular domain comprises the amino acid sequence of SEQ ID NO: 46, and wherein the nucleic acid molecule comprises the sequence of SEQ ID NO: 7 or 62.