	US 12,121,578 B2
	RSV F/G chimeric vaccine
Ryo Yamaue, Kikuchi (JP); Satoshi Kamakura, Kikuchi (JP); Mihoko Matsuo, Kikuchi (JP); Masaharu Torikai, Kikuchi (JP); and Hiroaki Mori, Kikuchi (JP)
Assigned to KM BIOLOGICS CO., LTD., Kumamoto (JP)
Appl. No. 17/431,833
Filed by KM Biologics Co., Ltd., Kumamoto (JP)
PCT Filed Feb. 27, 2020, PCT No. PCT/JP2020/008187 § 371(c)(1), (2) Date Aug. 18, 2021, PCT Pub. No. WO2020/175660, PCT Pub. Date Sep. 3, 2020.
Claims priority of application No. 2019-036206 (JP), filed on Feb. 28, 2019.
Prior Publication US 2022/0111035 A1, Apr. 14, 2022
Int. Cl. A61K 39/155 (2006.01); A61P 31/14 (2006.01); C12N 7/00 (2006.01); C12N 15/62 (2006.01)

CPC A61K 39/155 (2013.01) [A61P 31/14 (2018.01); C12N 7/00 (2013.01); C12N 15/62 (2013.01)]

27 Claims

1. A chimeric protein (RSV F/G protein) of Respiratory Syncytial Virus (RSV) F protein and G protein, the chimeric protein comprising an amino acid sequence of the RSV F protein having an identity of 90% or more to the amino acid sequence of SEQ ID NO: 1 (the RSV F protein), and a portion of CCD sequence of RSV G protein,

wherein a portion of the RSV F protein is replaced with a portion of CCD sequence of RSV G protein or wherein a portion of CCD sequence of RSV G is added to the RSV F protein,

the RSV F protein is composed of sites including an SP domain, an F2 domain, a p27 domain, an FP domain, an F1 domain, in order from an N-terminus, and

wherein the portion of the RSV F protein that is replaced with a portion of CCD sequence of RSV G protein is selected from the group consisting of positions 137 to 146 of SEQ ID NO: 1; positions 382 to 393 of SEQ ID NO: 1; and positions 425 to 436 of SEQ ID NO: 1; and

wherein:

(1) the portion of the CCD sequence to be replaced or added comprises a sequence selected from the group consisting of a sequence at positions 162 to 171 of SEQ ID NO: 2, a sequence at positions 187 to 197 of SEQ ID NO: 2, a sequence in which sequences at positions 162 to 172 and at positions 187 to 199 of SEQ ID NO: 2 are linked, a sequence in which sequences at positions 164 to 172 and at positions 187 to 197 of SEQ ID NO: 2 are linked, a sequence in which sequences at positions 162 to 172, at positions 187 to 199 and at positions 162 to 172 of SEQ ID NO: 2 are linked, and a sequence in which two or three of a sequence at positions 162 to 172 of SEQ ID NO: 2 are linked, or

(2) the portion of the CCD sequence to be replaced or added comprises a sequence selected from the group consisting of a sequence at positions 162 to 171 of SEQ ID NO: 11 of RSV G protein, a sequence at positions 164 to 172 of SEQ ID NO: 11 of RSV G protein, and a sequence at positions 187 to 197 of SEQ ID NO: 11 of RSV G protein, a sequence in which sequences at positions 162 to 172 and at positions 187 to 197 of SEQ ID NO: 11 are linked, a sequence in which sequences at positions 164 to 172 and at positions 187 to 197 of SEQ ID NO: 11 are linked, a sequence in which sequences at positions 162 to 172 and at positions 187 to 199 of SEQ ID NO: 11 are linked, and a sequence in which two or three of a sequence at positions 162 to 172 of SEQ ID NO: 11 are linked.