US 12,120,996 B2
Induction method for improving yield and fertility of 2n pollen in Camellia oleifera
Zhiqiang Han, Changsha (CN); Hongda Deng, Changsha (CN); Xiaoyu Zhang, Changsha (CN); Hailang Tong, Changsha (CN); Xing Liu, Changsha (CN); and Tongyue Zhang, Changsha (CN)
Assigned to CENTRAL SOUTH UNIVERSITY OF FORESTRY & TECHNOLOGY, Changsha (CN)
Filed by Central South University of Forestry and Technology, Changsha (CN)
Filed on Jan. 9, 2024, as Appl. No. 18/407,908.
Prior Publication US 2024/0237599 A1, Jul. 18, 2024
Int. Cl. A01H 1/08 (2006.01)
CPC A01H 1/08 (2013.01) 5 Claims
 
1. A method for improving yield and fertility of 2n pollen in Camellia oleifera, said method comprising:
1) treating flower buds of a Camellia oleifera plant with a 5 mg/L to 20 mg/L N,N′-dimethylthiourea (DMTU)-containing solution by the following process:
when flower buds of a Camellia oleifera plant comprise styles 1.97 mm to 2.17 mm in length:
(a) spraying a flower branch of the Camellia oleifera plant with the DMTU-containing solution; or
(b) injecting the DMTU-containing solution into flower buds on a flower branch of the Camellia oleifera plant; and
2) heat treating the flower branch at 45° C. to 47° C. for 4 hours to 5 hours; wherein the DMTU-containing solution further comprises: 0.1 g/L to 2.0 g/L mannitol, 0.5 mg/L to 2.0 mg/L ascorbic acid, 2.0 mg/L to 8.0 mg/L brassinolide, and 5.0 g/L to 30.0 g/L sucrose.